Purification and biochemical characterization of an extracellular lipase from psychrotolerant Pseudomonas fluorescens KE38
نویسندگان
چکیده
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منابع مشابه
EXTRACELLULAR LIPA SE ACTIVITY CHARACTERIZATION OF SOME PSEUDOMONAS AERUGINOSA STRA INS ISOLATED FROM HUMAN INFECTIONS
Pseudomonas aeruginosa EF2, ATCC 9027 and ATCC 19660 were grown in a continuous culture under Tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (pH 6.5, 37°C at dilution rate of 0.05/h). Culture supernatants were carefully removed and stored at -20°C. To purify the lipases, the culture supernatant was reduced in volume to approximately 10 mL by an ultrafiltra...
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